5ZME

Nucleotide-free form of C. reinhardtii ArsA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-25
Detector	RAYONIX MX300HE
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	91.035, 91.035, 307.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.861 - 3.603
R-factor	0.2911
Rwork	0.288
R-free	0.31910
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.606
Data scaling software	HKL-2000
Phasing software	PHENIX ((1.11_2567: ???))
Refinement software	PHENIX ((1.11_2567: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.860	3.730
High resolution limit [Å]	3.600	3.600
Number of reflections	15761
<I/σ(I)>	29.9
Completeness [%]	99.5
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	100 mM Sodium HEPES pH7.5, 20% Glycerol, 10% PEG6000 and 3% 2-Methyl-2,4-pentanediol