5ZLV
DNA polymerase IV - DNA ternary complex with 50mM MgCl2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.310, 57.240, 110.390 |
| Unit cell angles | 90.00, 94.74, 90.00 |
Refinement procedure
| Resolution | 50.778 - 2.350 |
| R-factor | 0.1915 |
| Rwork | 0.188 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ir1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.009 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.200 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 45186 | 6322 |
| <I/σ(I)> | 9.3 | 2 |
| Completeness [%] | 98.4 | 96.7 |
| Redundancy | 3.6 | 3.2 |
| CC(1/2) | 0.995 | 0.736 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 15% MPD , pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
