5ZLI

Crystal structure of glutamine synthetase from helicobacter pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97950
Spacegroup name	C 1 2 1
Unit cell lengths	191.085, 131.462, 131.411
Unit cell angles	90.00, 122.67, 90.00

Refinement procedure

Resolution	19.996 - 2.800
R-factor	0.192
Rwork	0.190
R-free	0.26010
RMSD bond length	0.011
RMSD bond angle	1.278
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.800	7.590	2.800
Rmerge	0.117	0.062	0.431
Rmeas	0.140	0.074	0.513
Rpim	0.076	0.040	0.276
Total number of observations	224008
Number of reflections	67460	3434	3364
<I/σ(I)>	7
Completeness [%]	99.5	98.6	99.8
Redundancy	3.3	3.3	3.4
CC(1/2)		0.991	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	296	PEG 6000, choline, Hepes