5ZKS
Crystal structure of DFA-IIIase from Arthrobacter chlorophenolicus A6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-07-30 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 6 |
| Unit cell lengths | 134.348, 134.348, 78.554 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.370 - 2.000 |
| R-factor | 0.11091 |
| Rwork | 0.109 |
| R-free | 0.13948 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2inu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.462 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 54535 | |
| <I/σ(I)> | 21.8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | 10% PEG 3350, 0.1M Sodium malonate (pH 4.2) |
