5ZKH
Crystal Structure of N-terminal Domain of Plasmodium falciparum p43
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-02-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.710, 44.371, 77.472 |
| Unit cell angles | 90.00, 111.94, 90.00 |
Refinement procedure
| Resolution | 36.464 - 2.991 |
| R-factor | 0.2161 |
| Rwork | 0.214 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 8.130 | 3.000 |
| Rmerge | 0.081 | 0.031 | 0.552 |
| Total number of observations | 43159 | ||
| Number of reflections | 7156 | 391 | 337 |
| <I/σ(I)> | 10.5 | ||
| Completeness [%] | 99.8 | 100 | 98.8 |
| Redundancy | 6 | 5.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350 |
