5ZKG

Crystal Structure of C-terminal Domain of Plasmodium vivax p43

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980
Spacegroup name	P 43
Unit cell lengths	105.200, 105.200, 36.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.047 - 3.300
R-factor	0.2197
Rwork	0.216
R-free	0.27780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ntg
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.050	3.500
High resolution limit [Å]	3.300	3.300
Number of reflections	6367	993
<I/σ(I)>	5.92	0.86
Completeness [%]	99.8	98.8
Redundancy	12.8	10.3
CC(1/2)	0.990	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M bicine/Trizma base pH 8.5, 0.02M of alcohols [0.2M 1,6-hexanediol, 0.2M 1-butanol, 0.2M (RS)-1,2propanediol, 0.2M 2-propanol, 0.2M 1,4-butanediol, 0.2M 1,3-propanediol], 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD