5ZK6

Crystal structure of RimK complexed with RpsF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-04
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.000000
Spacegroup name	P 64 2 2
Unit cell lengths	131.272, 131.272, 168.571
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.969 - 3.150
R-factor	0.2095
Rwork	0.208
R-free	0.24490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zct
RMSD bond length	0.007
RMSD bond angle	1.155
Data reduction software	XDS
Data scaling software	Aimless (0.5.17)
Phasing software	PHASER (2.8.0)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.130	47.130	3.360
High resolution limit [Å]	3.150	8.900	3.150
Rmerge	0.167	0.037	2.942
Rmeas	0.170	0.037	2.981
Rpim	0.028	0.007	0.478
Number of reflections	15497	793	2724
<I/σ(I)>	19.9
Completeness [%]	99.9	99.3	99.6
Redundancy	37.7	29.3	38.2
CC(1/2)	1.000	1.000	0.926

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100mM Tris-HCl (pH 7.8), 600mM Sodium formate, 8% Poly-gamma-glutamic acid 200-400