5ZJZ
Stapled-peptides tailored against initiation of translation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.978570 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.270, 38.300, 78.930 |
| Unit cell angles | 90.00, 122.74, 90.00 |
Refinement procedure
| Resolution | 45.590 - 1.670 |
| R-factor | 0.19068 |
| Rwork | 0.190 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tpw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.496 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Number of reflections | 26192 | |
| <I/σ(I)> | 14.28 | 2.26 |
| Completeness [%] | 97.6 | 94.4 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 12.5%(w/v) PEG20000, 20%(w/v) PEG MME 550, 0.1M MES/imidazole pH 6.5 |
