5ZJQ
Structure of AbdB/Exd complex bound to a 'Red14' DNA sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979180 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.056, 49.440, 95.194 |
| Unit cell angles | 90.00, 109.33, 90.00 |
Refinement procedure
| Resolution | 29.942 - 2.443 |
| R-factor | 0.2259 |
| Rwork | 0.223 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b8i |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.903 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.942 | 2.530 |
| High resolution limit [Å] | 2.443 | 2.443 |
| Rmerge | 0.065 | 0.539 |
| Number of reflections | 12413 | 980 |
| <I/σ(I)> | 18.49 | |
| Completeness [%] | 97.3 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 200mM MgCl2, 0.1M Tris pH 5.8, 17.5% PEG 4000, 5% glycerol |
