5ZJL
Crystal Structure of the dust mite allergen Der f 23 from Dermatophagoides farinae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 39.049, 39.048, 109.970 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 21.990 - 1.700 |
| R-factor | 0.28712 |
| Rwork | 0.286 |
| R-free | 0.30091 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zce |
| RMSD bond length | 0.042 |
| RMSD bond angle | 2.980 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 15991 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 87.0 | |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M MgCl2, 0.1 Sodium Citrate (pH 5.0), 2% PEG2000 |
