5ZJ5

Guanine-specific ADP-ribosyltransferase with NADH and GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	116.197, 40.049, 77.054
Unit cell angles	90.00, 113.97, 90.00

Refinement procedure

Resolution	27.149 - 1.568
R-factor	0.1751746266
Rwork	0.174
R-free	0.20841
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5daz
RMSD bond length	0.013
RMSD bond angle	1.444
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.150	1.624
High resolution limit [Å]	1.568	1.568
Rmeas	0.067	0.422
Rpim	0.028	0.183
Number of reflections	45603	4374
<I/σ(I)>	24.54	4.53
Completeness [%]	99.6	96.58
Redundancy	5.5	5
CC(1/2)	0.998	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	ammonium acetate, PEG 10000