5ZJ4

Guanine-specific ADP-ribosyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	93.048, 51.185, 94.972
Unit cell angles	90.00, 116.24, 90.00

Refinement procedure

Resolution	35.637 - 1.497
R-factor	0.162708839954
Rwork	0.162
R-free	0.18502
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5daz
RMSD bond length	0.015
RMSD bond angle	1.359
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.640	1.551
High resolution limit [Å]	1.497	1.497
Rmeas	0.106	0.903
Rpim	0.040	0.337
Number of reflections	129121	12711
<I/σ(I)>	18.28	2.12
Completeness [%]	99.8	98.73
Redundancy	7.2	7.1
CC(1/2)	0.998	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	sodium acetate, MPD