5ZJ3

Textilinin-1, A Kunitz-Type Serine Protease Inhibitor From pichia expression system

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-01
Detector	RAYONIX SX-165mm
Wavelength(s)	1.5418
Spacegroup name	I 4 2 2
Unit cell lengths	98.752, 98.752, 80.206
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.686 - 1.880
R-factor	0.1897
Rwork	0.188
R-free	0.22310
RMSD bond length	0.007
RMSD bond angle	1.104
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.910
High resolution limit [Å]	1.880	1.880
Number of reflections	16431	808
<I/σ(I)>	6.4
Completeness [%]	100.0	100
Redundancy	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	0.1M Na HEPES, 2%(v/v) PEG 400, Mammonium sulfate, pH 7.5