5ZJ0
Crystal structure of Xanthomonas campestris FlgL (space group C2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Collection date | 2017-07-25 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00004 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.182, 63.695, 51.284 |
| Unit cell angles | 90.00, 105.16, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.20871 |
| Rwork | 0.207 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ziz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.211 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.071 | 0.386 |
| Number of reflections | 24596 | 1209 |
| <I/σ(I)> | 25 | 3.3 |
| Completeness [%] | 98.7 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 19% PEG6000, 0.1M Hepes, pH 7.0, and 1.0M lithium chloride |
