5ZIZ
Crystal structure of Xanthomonas campestris FlgL (space group H3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-28 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00004 |
| Spacegroup name | H 3 |
| Unit cell lengths | 179.372, 179.372, 54.755 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.21082 |
| Rwork | 0.208 |
| R-free | 0.25795 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.268 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.083 | 0.516 |
| Number of reflections | 33440 | 1673 |
| <I/σ(I)> | 21.9 | 3.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 17% PEG 3350, 0.3M magnesium nitrate |
