5ZIU
Crystal structure of human Entervirus D68 RdRp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97775 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.835, 59.835, 358.923 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.962 - 2.147 |
| R-factor | 0.2142 |
| Rwork | 0.211 |
| R-free | 0.26640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xe0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.090 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 37191 | 3002 |
| <I/σ(I)> | 33.5 | 7 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 25.8 | 25.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | 0.1M Tris, PH 8.0, 3.5M NH4Cl |
