5ZIT
Crystal structure of human Enterovirus D68 RdRp in complex with NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97775 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 60.683, 60.683, 360.518 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.428 - 3.196 |
| R-factor | 0.2509 |
| Rwork | 0.248 |
| R-free | 0.31420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xe0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.143 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.280 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Rmerge | 0.281 | 0.631 |
| Number of reflections | 12153 | 948 |
| <I/σ(I)> | 12.67 | 6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 23.8 | 25.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | 0.1M Tris, PH 8.0, 3.5M NH4Cl, 10mM Chromic chroride hexahydrate,5mM NADPH |
