5ZIB

Crystal structure of human GnT-V luminal domain in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-25
Detector	RAYONIX MX-300
Wavelength(s)	0.9
Spacegroup name	P 65 2 2
Unit cell lengths	97.671, 97.671, 268.856
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.049 - 1.900
R-factor	0.1911
Rwork	0.189
R-free	0.22430
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.875
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.820	2.000
High resolution limit [Å]	1.900	1.900
Rmeas	0.253
Rpim	0.066	0.494
Number of reflections	60787
<I/σ(I)>	8.8	2.5
Completeness [%]	100.0	100
Redundancy	14.6	14.8
CC(1/2)	0.995	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.15M DL-malic acid (pH 7.0), 20% (w/v) polyethylene glycol (PEG) 3350