5ZI2
MDH3 wild type, nad-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-27 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.099, 92.559, 82.114 |
| Unit cell angles | 90.00, 99.88, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.17802 |
| Rwork | 0.175 |
| R-free | 0.22977 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mld |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.947 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rpim | 0.048 | 0.329 |
| Number of reflections | 44896 | |
| <I/σ(I)> | 30.4 | 4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M Bis-Tris pH 5.5, 0.2M MgCl2, 25% (w/v) PEG 3350 |
