5ZHA
Structure of Y68F mutant Mn-Bound periplasmic metal binding protein from candidatus liberibacter asiaticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 93.952, 93.952, 94.567 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.800 - 1.840 |
| R-factor | 0.14248 |
| Rwork | 0.140 |
| R-free | 0.18905 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4udn |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.858 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.800 | 1.870 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 45940 | |
| <I/σ(I)> | 23.5 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.4 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 277.15 | 0.1M SODIUM ACETATE TRIHYDRATE pH 4.6, 2M AMMONIUM SULPHATE |
