5ZH8
Crystal Structure of FmtA from Staphylococcus aureus at 2.58 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 105.391, 128.234, 107.599 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.20993 |
| Rwork | 0.208 |
| R-free | 0.25436 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gdn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0216) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.620 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Number of reflections | 22784 | |
| <I/σ(I)> | 13.28 | 2.1 |
| Completeness [%] | 95.7 | 28 |
| Redundancy | 3.4 | 2 |
| CC(1/2) | 0.980 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M NaCl, 0.1 M Tris pH 8.5, 25% PEG 3350 |
