5ZGN
The crystal structure of KacTA-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.046, 62.044, 61.994 |
| Unit cell angles | 93.83, 93.99, 93.93 |
Refinement procedure
| Resolution | 28.440 - 2.240 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.832 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.330 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.076 | 0.311 |
| Number of reflections | 38226 | |
| <I/σ(I)> | 19.4 | |
| Completeness [%] | 94.2 | 92.1 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.05M ammonium sulfate, 0.05M BIS-TRIS pH 6.5, 30% pentaerythritol ethoxylate (15/4 EO/OH) |
