5ZGL
hnRNP A1 segment GGGYGGS (residues 234-240)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-06-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 11.957, 10.141, 21.372 |
Unit cell angles | 90.00, 100.11, 90.00 |
Refinement procedure
Resolution | 11.771 - 0.950 |
R-factor | 0.102 |
Rwork | 0.100 |
R-free | 0.12110 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.193 |
Data scaling software | XSCALE |
Phasing software | SHELXD |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 11.771 | 11.774 | 0.980 |
High resolution limit [Å] | 0.950 | 4.250 | 0.950 |
Rmerge | 0.054 | 0.179 | |
Rmeas | 0.064 | 0.218 | |
Number of reflections | 5179 | 55 | 178 |
<I/σ(I)> | 13.59 | 19.24 | 6.64 |
Completeness [%] | 78.6 | 36.2 | |
Redundancy | 3.267 | 3.145 | 3.017 |
CC(1/2) | 0.997 | 0.962 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.2 M ammonium sulfate, 30% PEG 4000 |