5ZGL

hnRNP A1 segment GGGYGGS (residues 234-240)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-06-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	11.957, 10.141, 21.372
Unit cell angles	90.00, 100.11, 90.00

Refinement procedure

Resolution	11.771 - 0.950
R-factor	0.102
Rwork	0.100
R-free	0.12110
RMSD bond length	0.012
RMSD bond angle	1.193
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	11.771	11.774	0.980
High resolution limit [Å]	0.950	4.250	0.950
Rmerge		0.054	0.179
Rmeas		0.064	0.218
Number of reflections	5179	55	178
<I/σ(I)>	13.59	19.24	6.64
Completeness [%]		78.6	36.2
Redundancy	3.267	3.145	3.017
CC(1/2)		0.997	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.2 M ammonium sulfate, 30% PEG 4000