5ZGD

hnRNPA1 reversible amyloid core GFGGNDNFG (residues 209-217) determined by X-ray

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-10-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	4.869, 27.263, 35.311
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.580 - 1.401
R-factor	0.1255
Rwork	0.122
R-free	0.15930
RMSD bond length	0.007
RMSD bond angle	0.802
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.580	1.490
High resolution limit [Å]	1.400	1.400
Rmerge	0.132	0.367
Rmeas		0.433
Number of reflections	1676	377
<I/σ(I)>	7.83	3.01
Completeness [%]	89.1	65.6
Redundancy	7.1	3.3
CC(1/2)	0.990	0.831

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	ammonium sulfate, HEPES