5ZG9

Crystal structure of MoSub1-ssDNA complex in phosphate buffer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	I 1 2 1
Unit cell lengths	42.010, 80.190, 76.910
Unit cell angles	90.00, 91.88, 90.00

Refinement procedure

Resolution	40.095 - 2.040
R-factor	0.1788
Rwork	0.176
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.264
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.095	2.113
High resolution limit [Å]	2.040	2.040
Number of reflections	15521
<I/σ(I)>	22.11
Completeness [%]	95.3
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	1.8 M K2HPO4-NaH2PO4 pH 7.5