5ZFX
Crystal Structure of Triosephosphate isomerase from Opisthorchis viverrini
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2013-11-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00001 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 96.529, 206.621, 97.466 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.075 - 1.751 |
| R-factor | 0.1648 |
| Rwork | 0.164 |
| R-free | 0.20040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tph |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.929 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.105 | 0.114 | 0.110 |
| Total number of observations | 541897 | ||
| Number of reflections | 96825 | 10127 | 9052 |
| <I/σ(I)> | 37.7 | ||
| Completeness [%] | 98.8 | 99.6 | 93.3 |
| Redundancy | 5.6 | 8.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 293 | PEG 3350 |
