5ZFP

Structure of the ExbB/ExbD hexameric complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	90
Detector technology	PIXEL
Collection date	2016-10-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	121.470, 106.340, 163.360
Unit cell angles	90.00, 111.75, 90.00

Refinement procedure

Resolution	50.180 - 2.840
R-factor	0.247
Rwork	0.245
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5sv0
RMSD bond length	0.010
RMSD bond angle	1.110
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.180	2.940
High resolution limit [Å]	2.840	2.840
Rmerge	0.110	0.500
Rmeas	0.155	0.706
Number of reflections	90711
<I/σ(I)>	6.67
Completeness [%]	99.3
Redundancy	5
CC(1/2)	0.992	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	0.1 M glycine, pH 9.0, 0.15 M CaCl2, 40 % PEG 350 MME, 0.05-0.2 M L-arginine