5ZE7

UDP Glucose alpha tetrahydrobiopterin glycosyltransferase from Synechococcus species PCC 7942 - apo form

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-28
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979, 0.979,0.964
Spacegroup name	C 1 2 1
Unit cell lengths	171.350, 77.992, 53.770
Unit cell angles	90.00, 90.27, 90.00

Refinement procedure

Resolution	45.447 - 1.990
R-factor	0.1872
Rwork	0.185
R-free	0.22200
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.043
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.990	1.990
Number of reflections	48642
<I/σ(I)>	8.6
Completeness [%]	99.9
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Bis-tris, PEG 3350, Galactose