5ZDI
Crystal structure of CsaA chaperone protein from picrophilus torridus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-12-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.009, 65.334, 75.588 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.430 - 1.700 |
| R-factor | 0.18496 |
| Rwork | 0.183 |
| R-free | 0.22361 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nzh |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.027 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.6.1) |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 25764 | |
| <I/σ(I)> | 24.96 | |
| Completeness [%] | 98.0 | |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 20mM Tris buffer pH 8.0, 0.2 M ammonium sul-phate, 1 M NaCl, 25% PEG 8000 |
