5ZDF
Crystal structure of poly(ADP-ribose) glycohydrolase (PARG) T267K mutant from Deinococcus radiodurans in complex with ADP-ribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-03 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.99984 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.176, 63.176, 130.929 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.449 - 2.504 |
| R-factor | 0.2044 |
| Rwork | 0.199 |
| R-free | 0.24870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.449 | 2.590 |
| High resolution limit [Å] | 2.504 | 2.500 |
| Rmerge | 0.147 | 0.508 |
| Number of reflections | 10823 | |
| <I/σ(I)> | 15.4 | 2.259 |
| Completeness [%] | 98.8 | 90.4 |
| Redundancy | 6.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.1 M Sodium citrate tribasic dihydrate pH 5.2, 20 % 2-Propanol, 20% Polyethylene glycol 4000, 10 mM Hexammine cobalt (lll) chloride |
