5ZDE
Crystal structure of poly(ADP-ribose) glycohydrolase (PARG) from Deinococcus radiodurans in complex with ADP-ribose (P3221)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 62.894, 62.894, 131.254 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.230 - 2.800 |
R-factor | 0.1903 |
Rwork | 0.182 |
R-free | 0.26870 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.988 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | phenix.refine (1.13_2998) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.234 |
High resolution limit [Å] | 2.803 |
Rmerge | 0.088 |
Number of reflections | 7430 |
<I/σ(I)> | 30.11 |
Completeness [%] | 99.9 |
Redundancy | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 20 % 2-Propanol, 20% Polyethylene glycol 4000 |