5ZCY
Crystal structure of archaeal translation initiation factor 1 at 1.5 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-10-26 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 53.580, 53.580, 56.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.970 - 1.500 |
| R-factor | 0.1753 |
| Rwork | 0.174 |
| R-free | 0.20460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mo0 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.578 |
| Data reduction software | MOSFLM (7.2.1) |
| Data scaling software | Aimless (0.5.28) |
| Phasing software | PHASER (2.6.1) |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.970 | 1.520 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.054 | 0.514 |
| Rmeas | 0.056 | 0.540 |
| Rpim | 0.017 | 0.165 |
| Number of reflections | 14801 | 691 |
| <I/σ(I)> | 23.4 | |
| Completeness [%] | 98.1 | 95.9 |
| Redundancy | 11 | 10.3 |
| CC(1/2) | 1.000 | 0.915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 0.2M Lithium Nitrate, 20% PEG 3350 |
