5ZCU

Crystal structure of RCAR3:PP2C wild-type with pyrabactin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	75.036, 134.577, 190.267
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.496 - 2.413
R-factor	0.2095
Rwork	0.207
R-free	0.25030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gwp
RMSD bond length	0.004
RMSD bond angle	0.843
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.13_2998)
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.496	2.538
High resolution limit [Å]	2.450	2.450
Rmerge	0.097	0.590
Rpim	0.049	0.298
Number of reflections	36217	3212
<I/σ(I)>	18.72
Completeness [%]	93.4
Redundancy	4.6
CC(1/2)	0.995	0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	PEG 3350, ammonium sulfate, sucrose