5ZCR
DSM5389 glycosyltrehalose synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.132, 84.567, 128.662 |
| Unit cell angles | 90.00, 103.75, 90.00 |
Refinement procedure
| Resolution | 70.039 - 2.400 |
| R-factor | 0.1746 |
| Rwork | 0.171 |
| R-free | 0.24780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iv8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.144 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.040 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 0.151 | 0.538 |
| Number of reflections | 54626 | 5332 |
| <I/σ(I)> | 7.4 | 2 |
| Completeness [%] | 93.9 | 92.3 |
| Redundancy | 2.6 | 2.5 |
| CC(1/2) | 0.698 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 8000, EDTA, phosphate |
