5ZCL

Crystal structure of OsPP2C50 I267L:OsPYL/RCAR3 with (+)-ABA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	75.096, 130.262, 80.252
Unit cell angles	90.00, 115.99, 90.00

Refinement procedure

Resolution	38.149 - 2.661
R-factor	0.1975
Rwork	0.195
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gwp
RMSD bond length	0.005
RMSD bond angle	0.706
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.149	2.757
High resolution limit [Å]	2.661	2.661
Rmerge	0.120	0.708
Rpim	0.066	0.411
Number of reflections	39539	3799
<I/σ(I)>	9.22
Completeness [%]	99.3
Redundancy	4.1
CC(1/2)	0.993	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	12% (w/v) PEG 8000, 0.3M Mg(OAc)2, 0.1M MES pH 6.0