5ZCK
Structure of the RIP3 core region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 4.813, 17.151, 29.564 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.830 - 1.271 |
| R-factor | 0.1386 |
| Rwork | 0.137 |
| R-free | 0.15250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2on9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.024 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.830 | 1.320 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmerge | 0.085 | |
| Number of reflections | 776 | |
| <I/σ(I)> | 54.5 | |
| Completeness [%] | 98.2 | 98.4 |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M magnesium chloride, 0.1 M sodium cacodylate, 20% (v/v) PEG 200 |
