5ZCJ
Crystal structure of complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.144, 103.922, 61.470 |
| Unit cell angles | 90.00, 95.46, 90.00 |
Refinement procedure
| Resolution | 19.917 - 2.004 |
| R-factor | 0.1912 |
| Rwork | 0.190 |
| R-free | 0.21890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.509 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2932)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.920 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 308418 | |
| <I/σ(I)> | 13.94 | |
| Completeness [%] | 97.3 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.7 M Ammonium tartrate, 0.1 M Sodium acetate pH 4.6 |
