5ZCH

Crystal structure of OsPP2C50 I267W:OsPYL/RCAR3 with (+)-ABA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	71.731, 76.028, 78.586
Unit cell angles	62.30, 73.09, 65.01

Refinement procedure

Resolution	24.788 - 2.474
R-factor	0.2072
Rwork	0.206
R-free	0.23030
RMSD bond length	0.003
RMSD bond angle	0.671
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.788	2.563
High resolution limit [Å]	2.474	2.474
Number of reflections	45783
<I/σ(I)>	15.16
Completeness [%]	96.9
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% v/v PEG 400, 5% w/v PEG 3000, 10% v/v glycerol, 0.1M HEPES pH7.5