5ZBY
Crystal structure of a [NiFe] hydrogenase maturation protease HycI from Thermococcus kodakarensis KOD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2016-10-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 50.377, 50.377, 146.167 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.628 - 1.591 |
| R-factor | 0.2032 |
| Rwork | 0.202 |
| R-free | 0.22330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e85 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.632 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.628 | 1.630 |
| High resolution limit [Å] | 1.591 | 1.591 |
| Rmerge | 0.049 | 0.623 |
| Number of reflections | 25807 | 1300 |
| <I/σ(I)> | 35.4 | |
| Completeness [%] | 98.1 | |
| Redundancy | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | HEPES (pH 7.5), 2-methyl-2,4-pentanediol |
