5ZBT

Structure of legume lectin-like domain from Entamoeba histolytica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-11-24
Detector	RIGAKU RAXIS IV++
Wavelength(s)	2.28977
Spacegroup name	P 21 21 21
Unit cell lengths	56.051, 57.174, 72.489
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.594 - 2.400
R-factor	0.1769
Rwork	0.175
R-free	0.21260
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.959
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CRANK2
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.930	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	10084
<I/σ(I)>	38.47
Completeness [%]	93.0
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	289	0.1M Bis-Tris, pH 6.5, 28%(w/v) PEG monomethyl ether 2000