5ZBS

Crystal Structure of Kinesin-3 KIF13B motor Y73C mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-12
Detector	ADSC QUANTUM 1
Wavelength(s)	0.9789
Spacegroup name	P 32
Unit cell lengths	107.311, 107.311, 91.599
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.440 - 2.203
R-factor	0.1739
Rwork	0.172
R-free	0.21980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbj
RMSD bond length	0.008
RMSD bond angle	0.977
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.083	0.678
Number of reflections	59423
<I/σ(I)>	13.2	2.4
Completeness [%]	99.6	99.9
Redundancy	4.2
CC(1/2)	0.998	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.5	298	0.1M Bis-Tris, 2.0M ammonium sulfate