5ZBM
Structure of glycolate oxidase containing FMN from Nicotiana benthamiana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-15 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 4 |
| Unit cell lengths | 144.230, 144.230, 129.823 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.610 - 2.800 |
| R-factor | 0.17162 |
| Rwork | 0.171 |
| R-free | 0.18815 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gox |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.267 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.255 |
| Number of reflections | 32743 | 1473 |
| <I/σ(I)> | 12 | 4.77 |
| Completeness [%] | 90.0 | 90.9 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 35%(w/v) MPD, 0.2M ammonium acetate, 0.1M HEPES pH 7.5 |
