5ZBA

Crystal structure of Rtt109-Asf1-H3-H4-CoA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 65 2 2
Unit cell lengths	111.903, 111.903, 332.715
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.825 - 3.500
R-factor	0.2454
Rwork	0.243
R-free	0.29450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zb9 2hue
RMSD bond length	0.003
RMSD bond angle	0.497
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.630
High resolution limit [Å]	3.500	7.530	3.500
Rmerge	0.177	0.058	0.527
Rmeas	0.196	0.065	0.585
Rpim	0.080	0.028	0.240
Total number of observations	85255
Number of reflections	16416	1840	1587
<I/σ(I)>	4.3
Completeness [%]	99.5	98.9	99.7
Redundancy	5.2	4.7	5.4
CC(1/2)		0.998	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	100mM sodium citrate, pH 5.0, 22% PEG 1500, 400mM sodium iodide