5ZB3

Dimeric crystal structure of ORF57 from KSHV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 64 2 2
Unit cell lengths	167.529, 167.529, 227.632
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.149 - 3.506
R-factor	0.2593
Rwork	0.257
R-free	0.30600
RMSD bond length	0.004
RMSD bond angle	0.854
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.560
High resolution limit [Å]	3.500	3.500
Rmerge	0.141	0.805
Rmeas	0.149	0.846
Rpim	0.046	0.259
Number of reflections	24306	1185
<I/σ(I)>	26.4	3.7
Completeness [%]	99.8	99.9
Redundancy	10.3	10.5
CC(1/2)	0.955	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.01M Magnesium chloride, 0.05M Hepes sodium pH 7.0, 4M Lithium chloride