5ZB3
Dimeric crystal structure of ORF57 from KSHV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 167.529, 167.529, 227.632 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.149 - 3.506 |
| R-factor | 0.2593 |
| Rwork | 0.257 |
| R-free | 0.30600 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.854 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.560 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.141 | 0.805 |
| Rmeas | 0.149 | 0.846 |
| Rpim | 0.046 | 0.259 |
| Number of reflections | 24306 | 1185 |
| <I/σ(I)> | 26.4 | 3.7 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 10.3 | 10.5 |
| CC(1/2) | 0.955 | 0.948 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.01M Magnesium chloride, 0.05M Hepes sodium pH 7.0, 4M Lithium chloride |
