5ZAD
Human topoisomerase II beta in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-06 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97845 |
| Spacegroup name | P 32 |
| Unit cell lengths | 95.006, 95.006, 230.289 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.064 - 2.540 |
| R-factor | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qx3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.253 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.083 | 3.017 |
| Rmeas | 0.095 | 3.653 |
| Rpim | 0.045 | 2.021 |
| Number of reflections | 118178 | 5596 |
| <I/σ(I)> | 15.711 | 0.244 |
| Completeness [%] | 99.5 | 95.4 |
| Redundancy | 4.2 | 2.9 |
| CC(1/2) | 0.120 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100mM magnesium chloride, 50mM sodium citrate, pH 5.3, 20% MPD, 0.05% ethyl acetate |
