5Z9X
Arabidopsis SMALL RNA DEGRADING NUCLEASE 1 in complex with an RNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-04-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 87.993, 87.993, 178.639 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.986 - 2.800 |
| R-factor | 0.2527 |
| Rwork | 0.252 |
| R-free | 0.26140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SDN1 deltaC-ssRNA |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.843 |
| Data reduction software | HKL-2000 (0.98.699a) |
| Data scaling software | HKL-2000 (0.98.699a) |
| Phasing software | PHENIX (1.7.1_743) |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 20440 | |
| <I/σ(I)> | 26.6 | |
| Completeness [%] | 99.0 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | lithum sulfate, magnesium chloride, 2-(N-morpholino) ethanesulfonic acid |
