5Z9T
a new PL6 alginate lyase complex with trisaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2017-12-25 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 |
| Unit cell lengths | 106.086, 106.086, 101.894 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.16635 |
| Rwork | 0.165 |
| R-free | 0.19558 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5YJQ |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.785 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 118779 | |
| <I/σ(I)> | 26.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 288 | 2.4M sodium, malonate |
