5Z9S
Functional and Structural Characterization of a beta-Glucosidase Involved in Saponin Metabolism from Intestinal Bacteria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 277.15 |
| Detector technology | CCD |
| Collection date | 2016-12-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.954, 134.419, 90.285 |
| Unit cell angles | 90.00, 98.82, 90.00 |
Refinement procedure
| Resolution | 47.850 - 2.300 |
| R-factor | 0.2245 |
| Rwork | 0.224 |
| R-free | 0.24180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u48 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.732 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.850 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.134 | 0.652 |
| Number of reflections | 70856 | 6276 |
| <I/σ(I)> | 13.2 | 2.8 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 6.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | sodium formate, BICINE, polyethylene glycol monomethyl ether 5000 |
