5Z9A
Crystal structure of chorismate synthase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-03 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 76.530, 76.530, 389.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 66.280 - 2.790 |
| R-factor | 0.21027 |
| Rwork | 0.207 |
| R-free | 0.27602 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r53 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.604 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.280 | 2.891 |
| High resolution limit [Å] | 2.790 | 2.791 |
| Rmerge | 0.132 | 0.430 |
| Number of reflections | 17307 | 887 |
| <I/σ(I)> | 11.05 | |
| Completeness [%] | 96.4 | |
| Redundancy | 5.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 10mM HEPES pH 7.5, 8% PEG 3350 |
