5Z91

Human mitochondrial ferritin mutant bound with gold ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9789
Spacegroup name	F 4 3 2
Unit cell lengths	182.094, 182.094, 182.094
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.520 - 3.004
R-factor	0.1874
Rwork	0.182
R-free	0.28110
RMSD bond length	0.010
RMSD bond angle	1.102
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.520	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	5579
<I/σ(I)>	3.2
Completeness [%]	100.0
Redundancy	16.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	Bicine, MgCl2